Anti-Dicer Rabbit mAb

Product

Anti-Dicer Rabbit mAb

Catalog number :AT0762

Background:

Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha, pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs. The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA.

UniProt ID: Q9UPY3

Overview

Reactivity: Human, mouse, rat, bovine, pig

Tested applications: Western Blotting 1:1000

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP) 1:100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody recognizes endogenous levels of total Dicer protein.

Properties

Immunogen: a synthetic peptide corresponding to residues surrounding Ala1133 of human Dicer.

Clonality: Monoclonal, clone number: 3B7

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: Dicer Antibody - Lane 1: Marker [kDa]. Lane 2: Human cell line HEK293T

IHC Image: Immunohistochemistry-Paraffin: Dicer Antibody - Staining of human breast cancer (ductal carcinoma) shows moderate cytoplasmic positivity in cancer cells.

ICC/IF Image: Genetic Strategies Validation. Immunocytochemistry/Immunofluorescence: Dicer Antibody - Staining of A-431 cells using the anti-DICER1 antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.

Anti-Dicer Rabbit mAb

Related Products

Reviews