Anti-Doublecortin Rabbit mAb

Product

Anti-Doublecortin Rabbit mAb

Catalog number :AT0685

Mutations in Doublecortin cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation. Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain. Kinases JNK, CDK5 and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331 and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297. Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects.

Overview

Reactivity: Human, Mouse, Rat, Monkey

Tested applications: Western Blotting 1:1000

Immunoprecipitation 1:200

Immunohistochemistry (Paraffin) 1:500

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody detects endogenous levels of total Doublecortin protein.

Properties

Immunogen: Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Doublecortin.

Clonality: Monoclonal, clone number: 2B7

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: Doublecortin Antibody - Non-transfected (-) and Doublecortin-transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Doublecortin antibody at a dilution of 1:1000.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: Doublecortin Antibody - Staining of neurons derived from human ESC with a 1:200 dilution of anti-doublecortin antibody colocalizes with anti-MAP2 antibody (green).

Anti-Doublecortin Rabbit mAb

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