Anti-EGFR (phospho Y1068) Rabbit mAb

Product

Anti-EGFR (phospho Y1068) Rabbit mAb

Catalog number :AT0642

The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation. Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins. c-Src is involved in phosphorylation of EGFR at Tyr845. The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling. Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation. The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068. A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation. Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation.

Overview

Reactivity: Mouse, Rat, Human

Tested applications: Western Blot 1:1000

ELISA 1:100-1:2000

Flow Cytometry 1:100

Specificity: This antibody detects endogenous EGF receptor only when phosphorylated at Tyr1068. This antibody may cross-react weakly with other tyrosine-phosphorylated proteins.

Properties

Immunogen: Synthetic phosphopeptide (Human) derived from the region of EGFR that contains tyrosine 1068.

Clonality: Monoclonal, clone number: 4A1

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C

Host: Rabbit

Applications

WB Image: Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF for 5 minutes. PVDF membrane was probed with dilution(1:1000) of Phospho-EGFR (Y1068) rabbit Polyclonal Antibody (Catalog: AT0642), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog: AT0097). A specific band was detected for Phospho-EGFR (Y1068) at approximately 190 kDa (as indicated). This experiment was conducted under reducing condition.

Application Image: A431 human epithelial carcinoma cell line was untreated (open histogram) or treated for 5 minutes with 100 ng/mL Recombinant Human EGF then stained with dilution(1:300) of Phospho-EGFR (Y1068) rabbit Polyclonal Antibody (Catalog: AT0642), followed by Phycoerythrin-conjugated Anti-Rabbit IgG F(ab')2 Secondary Antibody,

To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Anti-EGFR (phospho Y1068) Rabbit mAb

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