Anti-ERp57 Rabbit mAb

Product

Anti-ERp57 Rabbit mAb - ER Marker

Catalog number :AT1604

Background:

Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds. This process is called oxidative protein folding. Disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds. Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum stress protein 57 (ERp57), constitute the PDI family. ERp57 interacts with calnexin and calreticulin and is suggested to play a role in the isomerization of disulfide bonds on certain glycoproteins.

UniProt ID: P30101

Overview

Reactivity: Human, Mouse, Rat, Bovine, Pig, Monkey

Tested applications: Western Blotting: 1/1000-1/2000

Immunofluorescence: 1/500

Immunohistochemistry (Paraffin): 1/500

Immunoprecipitation(IP) : 1/100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody detects endogenous levels of total ERp57 protein.

Properties

Immunogen: a synthetic peptide corresponding to residues surrounding Ala484 of human ERp57. Antibodies are purified by protein A and peptide affinity chromatography.

Clonality: Monoclonal, clone number: 6HA7

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: ERp57/PDIA3 Antibody - A. 20 ug rat liver lysate/extract 7.5 % SDS-PAGE ERp57 antibody, C-term dilution: 1:1000

IHC Image: Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody - Paraffin-embedded mouse lymph node. ERp57 antibody, C-term dilution: 1:500.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERp57 protein stained by ERp57 antibody, C-term diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining

Anti-ERp57 Rabbit mAb - ER Marker

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