Anti-ErbB4 (HER4) Rabbit mAb

Product

Anti-ErbB4 (HER4) Rabbit mAb

Catalog number :AT0645

Research studies have implicated the HER/ErbB receptor tyrosine kinase family in normal development, cardiac function and cancer. HER4/ErbB4, like other family members, has four ectodomains, a single transmembrane domain and a cytoplasmic tail containing the active tyrosine kinase domain. By binding to neuregulins and/or EGF family ligands, ErbB4 forms either a homodimer or heterodimer with other ErbB family members, which results in receptor activation and signaling. ErbB4 is ubiquitously expressed with the highest expression occurring in brain and heart. The expression of ErbB4 in breast cancer, pediatric brain cancer and other types of carcinomas has been reported in research studies suggesting that ErbB4 expression is involved in both normal tissue development and carcinogenesis.

Overview

Reactivity: Mouse, Rat, Human

Tested applications: Western Blotting 1:1000

Immunoprecipitation 1:100

Immunohistochemistry (Paraffin) 1:500

Immunofluorescence (Immunocytochemistry) 1:300

Flow Cytometry 1:200

Specificity: This antibody detects endogenous levels of ErbB4 protein. This antibody does not cross-react with other ErbB family members.

Properties

Immunogen: Recombinant fragment corresponding to Human ErbB 4 aa 1159-1308 (C terminal). Expressed in E. coli.

Sequence

Database link: Q15303

Clonality: Monoclonal, clone number: 5A32

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: ErbB4/Her4 Antibody - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

IHC Image: Immunohistochemistry-Paraffin: ErbB4/Her4 Antibody - Staining of human testis shows high expression.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: ErbB4/Her4 Antibody - Staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm.

Anti-ErbB4 (HER4) Rabbit mAb

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