Anti-Erk1 + Erk2 Rabbit mAb

Overview

Description

Rabbit monoclonal antibody to ERK1 + ERK2

Reactivity

Mouse, Rat, Human

Tested applications

IHC	: 1/50-1/200.
WB	: 1/500-1/2000. Predicted molecular weight: 42 and 44 kDa.
ICC/IF	: Use at an assay dependent concentration.
IP	: Use at 1 µg/mg of lysate.

Properties

Immunogen

Synthetic peptide, corresponding to C terminal amino acids 317-339 of Human ERK1 + ERK2.

Clonality

Monoclonal, clone number: 7B31

Isotype

Rabbit IgG

Form

Liquid
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3

Storage instruction

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Applications

IHC Image

 
AT0345 staining ERK1 + ERK2 in human stomach tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 23°C followed by incubation with the primary antibody at a 1/200 dilution for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal was used as secondary antibody.

ICC/IF Image

 AT0345 staining ERK1 + ERK2 in Embryonic Stem Cell-induced Mouse Neurospheres by Immunohistochemistry (Frozen sections). Sections were formaldehyde-fixed prior to blocking in 10% serum for 30 minutes at room temperature. The primary antibody was diluted 1/1000 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-Rabbit polyclonal antibody, diluted 1/1500 was used as the secondary.