Anti-FAK Rabbit mAb

Product

Anti-FAK Rabbit mAb

Catalog number :AT0375

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Overview

Description: Rabbit monoclonal antibody to FAK

Reactivity: Mouse, Rat, Human

Tested applications

WB	: 1/500 - 1/1000. Detects a band of approximately 125 kDa (predicted molecular weight: 119 kDa).
IHC-P	: 1/50 - 1/100.
ICC/IF	: 1/100 - 1/200.

Properties

Immunogen: Synthetic peptide conjugated to KLH, derived from around amino acids 923-927 (K-V-Y-E-N) of Human FAK (UniProt Q05397)

Clonality: Monoclonal, clone number: 6H7

Isotype: Rabbit IgG

Form: Liquid
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride

Storage instruction: Store at -20°C. Stable for 12 months at -20°C

Database links

Applications

WB Image

All lanes : Anti-FAK antibody (AT0375) at 1/500 dilution

Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with blocking peptide

Predicted band size : 119 kDa
Observed band size : 125 kDa

Why is the actual band size different from the predicted?

Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include...

post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein
post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases
splice variants - alternative splicing may create different sized proteins from the same gene
relative charge - the composition of amino acids (charged vs non-charged)
multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands

IHC Image: Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling FAK with AT0375 at 1/50 dilution. Right panel was preincubated with blocking peptide.

ICC/IF Image: Immunofluorescent analysis of methanol-fixed HeLa cells staining FAK with AT0375 at 1/100 dilution.

Anti-FAK Rabbit mAb

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