Anti-FGFR4 Rabbit mAb

Catalog number :AT0416

Receptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor. Binds FGF19.

Overview

Tested applications: WB: Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).

IHC-P: Use a concentration of 5 µg/ml.

ICC/IF: Use a concentration of 5 µg/ml.

Properties

Immunogen: Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human FGFR4.

Form: Liquid
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Database links

Applications

WB Image: Anti-FGFR4 antibody (AT0416) at 1 µg/ml + MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 89 kDa

Observed band size : 89 kDa

Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

IHC Image: IHC image of FGFR4 staining in Human liver cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with AT0416, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

ICC/IF Image: ICC/IF image of AT0416 stained human MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (AT0416, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells.