FLAG-Alpaca-Biotin Anti-DYKDDDDK (Flag) tag Alpaca Nanobody(VHH) Conjugated Biotin

Overview

Description

FLAG-Alpaca-Biotin Anti-DYKDDDDK (Flag) tag Alpaca Nanobody(VHH) Conjugated Biotin

Reactivity

Detect transfected DYKDDDDK tag fusion proteins expressed in E. coli, yeast, insect cell, mammalian cell, or in vitro transcription/translation systems. All systems that express DYKDDDDK tag fusion protein are suitable.

Tested applications

It can be coupled with streptavidin, or streptavidin-HRP,  or streptavidin-fluoresent dye, or streptavidin-beads.

Applications for which the Streptavidin-biotin interaction is used include:

 

Enzyme linked immunosorbent assay (ELISA)

Immunohistochemistry (IHC)

Western, Northern and Southern blotting

Immunoprecipitation

Cell surface labeling

Affinity purification

Fluorescence-activated cell sorting (FACS)

Electrophoretic mobility shift assays (EMSA)

 

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Product Picture

Product Picture 1

Figure 1. Alpaca antibodies are smaller than other mammalian antibodies. Mammalian IgG consists a heavy (green) and light (blue) chain, with variable heavy (VH) and variable light (VL) domains that facilitate the memory-like binding properties of antibodies. Camelid antibodies consist only a heavy chain (green) with a single variable heavy (VHH) domain for target recognition. This VHH domain can be expressed alone without the heavy chains, forming a single domain VHH.

Product Picture 2

Figure 2. Comparison of antibody types. Schematic representation of the comparison between (A) conventional antibodies, (B) alpaca heavy-chain antibodies, (C) and single domain antibodies.

Properties

Immunogen

Synthetic peptide: DYKDDDDK conjugated to KLH.

Clonality

Alpaca monoclonal nanobody(VHH,single domain antibody)

Form

Liquid

Storage instruction

Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Conjugation

conjugated biotin

Host

Alpaca pacous

Binding Capacity

Dissociation constant KD of 0.1nM

Storage buffer

In 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol with 0.02% (w/v) ProClin as preservative.

Purity

Recombinant expression in yeast and affinity purified

Applications

Application Image

Highlights

Benefits Of Alpaca nanobody
 

• No Fc fragment of ordinary antibodies, so there is no non-specific binding of Fc to the cell surface Fc receptor of immune cells in flow cytometry experiments
• Highly specific binding
• Low background
• High affinity
• High stability
• Alpaca nanobody is a small molecule with molecular weight of only 13kD (Conventional rabbit or mouse antibody is 150kD), so it can easily enter permeablized cells
• Animal-free production, recombinant expression in yeast and affinity purified