FLAG-Alpaca-HRP Anti-DYKDDDDK (Flag) tag Alpaca Nanobody(VHH) Conjugated HRP

Overview

Description

FLAG-Alpaca-HRP Anti-DYKDDDDK (Flag) tag Alpaca Nanobody(VHH) Conjugated HRP(Horseradish Peroxidase)

Reactivity

Detect transfected DYKDDDDK tag fusion proteins expressed in E. coli, yeast, insect cell, mammalian cell, or in vitro transcription/translation systems. All systems that express DYKDDDDK tag fusion protein are suitable.

Tested applications

WB: 1/5000-1/10000.

ELISA: 1/5000-1/10000.

 

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

There is no need to use secondary antibody

Product Picture

Product Picture 1

Figure 1. Alpaca antibodies are smaller than other mammalian antibodies. Mammalian IgG consists a heavy (green) and light (blue) chain, with variable heavy (VH) and variable light (VL) domains that facilitate the memory-like binding properties of antibodies. Camelid antibodies consist only a heavy chain (green) with a single variable heavy (VHH) domain for target recognition. This VHH domain can be expressed alone without the heavy chains, forming a single domain VHH.

 

Product Picture 3

Figure 2. Comparison of antibody types. Schematic representation of the comparison between (A) conventional antibodies, (B) alpaca heavy-chain antibodies, (C) and single domain antibodies.

Properties

Immunogen

Synthetic peptide: DYKDDDDK conjugated to KLH.

Clonality

Alpaca monoclonal nanobody(VHH,single domain antibody), without Fc fusion

Form

Liquid

Storage instruction

Store at –20 °C, (Avoid freeze / thaw cycles)

Conjugation

conjugated HRP(Horseradish Peroxidase)

Host

Alpaca pacous

Binding Capacity

Dissociation constant KD of 0.1nM

Storage buffer

In 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol, and 0.02% ProClin 300 as preservative. No BSA.

Purity

Recombinant expression in yeast and affinity purified

Applications

Highlights

Benefits Of Alpaca nanobody

 

No Fc fragment of ordinary antibodies, so there is no non-specific binding of Fc to the cell surface Fc receptor of immune cells in flow cytometry experiments
 

Highly specific binding
 

Low background
 

High affinity
 

High stability
 

Alpaca nanobody is a small molecule with molecular weight of only 13kD (Conventional rabbit or mouse antibody is 150kD), so it can easily enter permeablized cells
 

Animal-free production, recombinant expression in yeast and affinity purified