Anti-FOXP3 Rabbit mAb

Product

Anti-FOXP3 Rabbit mAb

Catalog number :AT0660

Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain. The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 - FoxP4, which are broadly expressed and play important roles in organ development, immune response and cancer pathogenesis. The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors.

FoxP2 regulates lung and nerve system development. Mutations, including point mutation, truncation, deletion, and chromosomal translocation, involving FoxP2 are associated with an inherited form of speech and language disorder.

Overview

Reactivity: Mouse, Rat, Human

Tested applications: Western Blotting 1:1000

Immunoprecipitation 1:200

Immunohistochemistry (Paraffin) 1:500

Flow Cytometry 1:50

Chromatin IP 1:100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)

Properties

Immunogen: Peptide with sequence SQRPSRCSNPTPGP corresponding to C-Terminus according to NP_054728.2, NP_001107849.1.

Clonality: Monoclonal, clone number: 5DA9

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: FoxP3 Antibody - (1:1000) staining of Human Muscle (A), MOLT4 (B) and negative control Pancreas (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

ICC/IF Image: Immunofluorescence: FoxP3 Antibody - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:500) followed by Alexa Fluor 488 secondary antibody, showing strong localization to nucleoplasm. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Anti-FOXP3 Rabbit mAb

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