Anti-Fibronectin Rabbit mAb

Product

Anti-Fibronectin Rabbit mAb

Catalog number :AT0663

Fibronectin EDA (FN-EDA, FN, fibronectin 1) is a glycoprotein that is involved in cell adhesion as well as cell migration processes such as embryogenesis, wound healing, host defense and metastasis. Fibronectin appears to be an important factor in the ability of cancerous cells to metastasize by inducing matrix metalloproteinases (MMPs). In a study of human laryngeal carcinoma cells, researchers showed that FN-induced expression of MMP-9 increased the DNA binding activity of transcription factors NF-kappa-B, Activator protein-1 and Specificity protein-1 to the promoter region (PMID: 20638438). Additionally, a study of renal cell cancer showed that the mRNA expression of fibronectin 1 was higher in patients with renal cell cancer as compared to patients with healthy kidneys. This disparity in mRNA expression levels could potentially be used to determine the aggressiveness of renal cell cancer as well as indicate the progression of the disease in patients (PMID: 20860816). Furthermore, a knockdown study of endogenous FN in the A431-III cell line showed a significant decrease of the migratory and invasive activity of the cells as well as an inhibition of secreted MMP-9 (PMID: 21036738).

Overview

Reactivity: Mouse, Rat, Human

Tested applications: Western Blotting 1:1000

Immunoprecipitation 1:200

Immunohistochemistry (Paraffin) 1:500

Flow Cytometry 1:50

Chromatin IP 1:100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody detects endogenous levels of total fibronectin protein.

Properties

Immunogen: A synthetic peptide made toward the C-terminal region of the human Fibronectin protein (within residues 2250-2300). [Swiss-Prot P02751]

Clonality: Monoclonal, clone number: 6N7

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: Fibronectin Antibody - WB analysis of Fibronectin in NIH 3T3 cell lysate.

IHC Image: Immunohistochemistry: Fibronectin Antibody - IHC analysis of Fibronectin in human renal cancer using DAB with hematoxylin counterstain.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: Fibronectin Antibody - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Fibronectin antibody at 1:500 overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Anti Alpha tubulin antibody was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Anti-Fibronectin Rabbit mAb

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