Flag-Alpaca-Nano™ Anti-DYKDDDDK (Flag) tag Alpaca Nanobody(VHH)

Overview

Description

Flag-Alpaca-Nano™ Anti-DYKDDDDK (Flag) tag Alpaca Nanobody(VHH)

Reactivity

Detect transfected DYKDDDDK tag fusion proteins expressed in E. coli, yeast, insect cell, mammalian cell, or in vitro transcription/translation systems. All systems that express DYKDDDDK tag fusion protein are suitable.

Product Picture 1

Figure 1. Alpaca antibodies are smaller than other mammalian antibodies. Mammalian IgG consists a heavy (green) and light (blue) chain, with variable heavy (VH) and variable light (VL) domains that facilitate the memory-like binding properties of antibodies. Camelid antibodies consist only a heavy chain (green) with a single variable heavy (VHH) domain for target recognition. This VHH domain can be expressed alone without the heavy chains, forming a single domain VHH.

Product Picture 2

Figure 2. Comparison of antibody types. Schematic representation of the comparison between (A) conventional antibodies, (B) alpaca heavy-chain antibodies, (C) and single domain antibodies.

Properties

Immunogen

Synthetic peptide: DYKDDDDK conjugated to KLH.

Clonality

Alpaca monoclonal nanobody(VHH,single domain antibody), without Fc fusion

Form

Liquid

Storage instruction

Store at –20 °C, (Avoid freeze / thaw cycles)

Conjugation

Unconjugated

Host

Alpaca pacous

Storage buffer

In 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol with 0.02% (w/v) ProClin as preservative.

Purity

Recombinant expression in yeast and affinity purified

Applications

Highlights

Benefits Of Alpaca nanobody

 

No Fc fragment of ordinary antibodies, so there is no non-specific binding of Fc to the cell surface Fc receptor of immune cells in flow cytometry experiments

 

Highly specific binding

 

Low background

 

High affinity

 

High stability

 

Alpaca nanobody is a small molecule with molecular weight of only 13kD (Conventional rabbit or mouse antibody is 150kD), so it can easily enter permeablized cells

 

Animal-free production, recombinant expression in yeast and affinity purified

Troubleshooting Guide

Attention

This VHH is not suitable for ELISA.

The binding of general anti VHH antibodies to nanobodies is unstable, with some nanobodies binding well and others not. Because the amino acid sequences of the skeleton FR1, FR2, FR3, FR4 of different nanobodies(VHH) are not completely consistent, and some have undergone site directed mutagenesis optimization, using anti VHH antibodies to identify nanobodies is NOT a good strategy.