CoIP, ChIP, CUT&RUN, CUT&Tag Expert! My Basket (...)
Worldwide delivery!
Having the Best Alpaca Nanobody Products!
Home | About us | Contact us | How to order |
    My Base Info
    Shopping Basket
    My Orders
    My Shipping Info
    Modify Password
    Log Out

    Anti-GAP43 Rabbit mAb - Neuronal Marker

    Catalog number :AT0675
    loading...
    GAP43 is a nervous system specific, growth-associated protein enriched in growth cones and areas of high plasticity. Phosphorylation of GAP43 at Ser41 by PKC is regulated by intracellular Ca2+ and affects the ability of GAP43 to bind calmodulin. GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex. Aberrant GAP43 expression can be seen in patients diagnosed with schizophrenia and Alzheimer's disease.
     
    GAP43 (growth associated protein 43, also called B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1) is a major component of motile "growth cones" that form the tips of elongating axons as well as the synaptic plasma membranes. GAP43 is associated with nerve growth process and plays a key role in axonal as well as dendritic filopodia induction. It has been detected in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN, and binds calmodulin with superior affinity in the absence of Ca2+ than in its presence. Intracellular Ca2+ regulates PKC mediated phosphorylation of GAP43 and affects GAP43's ability to bind calmodulin. GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex. Aberrant expression of GAP43 has been seen in patients diagnosed with schizophrenia and Alzheimer's disease. Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 was independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual SDS-PAGE mobility properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14 kDa (to perform such calculations yourself see this link). GAP43 is one of many highly negatively charged extended molecules which lack well defined tertiary structure and contain few hydrophobic residues and which run anomalously on SDS-PAGE. Other examples are CAP23, MARCKS, microtubule associated proteins MAP2, tau and the Neurofilament subunits. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.
     
    Overview
    Reactivity
    Human, Mouse, Rat, Monkey
    Tested applications
    Western Blotting 1:1000
    Immunoprecipitation 1:200
    Immunohistochemistry (Paraffin) 1:500
    Optimal dilutions/concentrations should be determined by the end user. 
     
    Specificity
    This antibody detects endogenous levels of total GAP43 protein.
    Properties
    Immunogen
    C-terminal peptide of rat and mouse GAP43, which is KEDPEADQEHA, with an N-terminal Cys added to allow chemical coupling to KLH carrier protein.
    Clonality
    Monoclonal, clone number: 4DS3
    Isotype
    Rabbit IgG
    Form
    Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol,0.02% Sodium Azide
    Storage instruction
    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.
    Host
    Rabbit
    Applications
    WB Image

    Western Blot: GAP-43 Antibody - Western blots of homogenate of cow cerebellum stained with RPCA-GAP-43. A prominent band running at ~43kDa represents the full length GAP-43.
    ICC/IF Image

    Immunocytochemistry/Immunofluorescence: GAP-43 Antibody - Cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to GAP43, dilution 1:2,000 in green, and costained with mouse mAb to vimentin, dilution 1:2,000, in red. Blue: DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while vimentin antibody stains intermediate filaments in fibroblasts and other non-neuronal cells.

    Related Products

    Reviews

    loading...
    Content *:
    Customer Review :
    Verification Code * :
    refresh image

    Shipping and Paying Info
    ......
    Order Information
    You can place an order online step by step as blow.

    Step 1. Register for a new account and Log in, Find out your product by searching our website.
    Step 2. Add the Product into your shopping basket, select your settlement currency.
    Step 3. Checkout by Paypal, Pay by credit card or bank transfer for your order.
    Step 4. Ship the goods for you, providing you package tracking numbers. 

    Any questions, please contact us via email: sale@engibodybio.com
    Payments by
    All our products are For Research Use Only. Not for diagnostic or therapeutic usages.