HA-Alpaca-HRP™ Anti-HA tag Alpaca Nanobody(VHH) Conjugated HRP

Overview

Description

HA-Alpaca-HRP™ Anti-HA tag Alpaca Nanobody(VHH) Conjugated HRP

Reactivity

Detect transfected HA tag fusion proteins expressed in E. coli, yeast, insect cell, mammalian cell, or in vitro transcription/translation systems. All systems that express HA tag fusion protein are suitable.

Tested applications

WB:1/5000-1/10000,
ELISA:1/5000-1/10000

 

There is no need to use secondary antibody

Properties

Immunogen

YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH.

Clonality

Alpaca monoclonal nanobody(VHH,single domain antibody), without Fc fusion

Form

Liquid

Storage instruction

Store at –20 °C, (Avoid freeze / thaw cycles)

Conjugation

Conjugated HRP(Horseradish Peroxidase)

Host

Alpaca pacous

Storage buffer

In 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol with 0.02% (w/v) ProClin as preservative.

Purity

Recombinant expression in yeast and affinity purified

Applications

Highlights

Benefits Of Alpaca Nanobody

 

No Fc fragment of ordinary antibodies, so there is no non-specific binding of Fc to the cell surface Fc receptor of immune cells in flow cytometry experiments

 

Highly specific binding

 

Low background

 

High affinity

 

High stability

 

Alpaca nanobody is a small molecule with molecular weight of only 13kD (Conventional rabbit or mouse antibody is 150kD), so it can easily enter permeablized cells

 

Animal-free production, recombinant expression in yeast and affinity purified