Anti-HDAC1 antibody - ChIP, CUT&RUN and CUT&Tag Grade

Overview

Description

Rabbit monoclonal  antibody to HDAC1

Reactivity

Rat, Human, human, Xenopus laevis, Zebrafish

Tested applications

IHC	: 1/300.
WB	: 1/1000-1/5000
ICC/IF	: 1/300-1/500

 ChIP, CUT&RUN and CUT&Tag

Properties

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of human HDAC1.

Clonality

Monoclonal

Isotype

Rabbit IgG

Form

Liquid
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Storage instruction

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Applications

WB Image

Anti-HDAC1 antibody (AT0360) at 1/3000 + whole embryo zebrafish tissue at 45 µg

Predicted band size : 56 kDa
Observed band size : 56 kDa
Additional bands at : 32 kDa (possible cross reactivity),37 kDa (possible cross reactivity).

ICC/IF Image

ICC/IF image of AT0360 stained human MCF7 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (AT0360, 1/300) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells.