Anti-HDAC2 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-HDAC2 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :AT0732

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription. HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes. In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity. Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases. Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents.

UniProt ID:

Q92769

Overview

Reactivity: Human, mouse, rat, bovine, chicken, Drosophilia melanogaster, Zebrafish

Tested applications: Western Blotting 1:1000

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP) 1;100
ChIP, CUT&RUN and CUT&Tag

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody recognizes endogenous levels of total HDAC2 protein. This antibody does not cross-react with HDAC1.

Properties

Immunogen: a synthetic peptide corresponding to C terminal human HDAC2

Clonality: Monoclonal, clone number: 4H21

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: Histone Deacetylase 2/HDAC2 Antibody - Analysis of HDAC2 on different lysates using anti-HDAC2 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: K562

ICC/IF Image: Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody - Staining HDAC2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Anti-HDAC2 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

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