Anti-HDAC4 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-HDAC4 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :AT0734

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription. HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes. In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity. Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases. Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents.

Histone deacetylase 4 (HDAC4), also known as HD4, KIAA0288, AHO3, BDMR, HDACA, and HA6116, is crucial for transcription regulation, cell cycle progression and developmental events. HDAC4 also plays a critical role in the deacetylation of lysine residues in the core histones, which results in the production of a tag for epigenetic repression. HDAC4 is a member of the class II histone deacetylase/AcuC/AphA family and has been linked to nucleosomal condensation. The HDAC4 protein is expressed ubiquitously throughout the body and can be found in both the nucleus and cytoplasm. HDAC4 gathers in the nuclei of myotubes during muscle cell differentiation, which indicates HDAC4 plays a role in muscle differentiation. HDAC4 is exported to the cytoplasm based on the interactions with a 14-3-3 chaperone protein and its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. Mutations in HDAC4 can cause brachydactyly-mental retardation syndrome (BDMR), which resembles Albright hereditary osteodystrophy. Recent research has found that HDAC4 regulates HIF-1, which influences cancer cell production and reaction to hypoxia making HDAC4 a possible protein of interest in cancer treatment (PMID: 21917920). Further research has found that HDAC4 affects the growth of cancerous cells in the prostate (PMID: 19013255). Other areas of research, involving knock-out studies, have found a link between HDAC4 and long term memory formation.

UniProt ID:

P56524

Overview

Reactivity: Human, mouse, rat, bovine, chicken, Drosophilia melanogaster, Zebrafish

Tested applications: Western Blotting 1:1000

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP) 1;100
ChIP, CUT&RUN and CUT&Tag

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody recognizes endogenous levels of total HDAC4 protein. The antibody may cross-react with HDAC5.

Properties

Immunogen: A synthetic peptide made to a C-terminal portion of the human HDAC4 protein (between residues 1000-1084)

Clonality: Monoclonal, clone number: 2B7

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Simple Western: Histone Deacetylase 4/HDAC4 Antibody - Simple Western lane view shows a specific band for HDAC4 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody - HDAC4 antibody was tested in HeLa cells at a 1:100 dilution against Dylight 488 (Green) using a 40X objective. Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.

Anti-HDAC4 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

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