Anti-HDAC8 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-HDAC8 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :AT0361

HDAC8 (Histone deacetylase 8) is a 50 kDa member of the class I subfamily, histone deacetylase family of transcriptional regulators. It is a smooth muscle enzyme that deacetylates histone lysines, blocking gene transcription. HDAC8 appears to function as a monomer, and phosphorylation at Ser39 inhibits activity. Human HDAC8 is 377 amino acids (aa) in length. It contains a series of alpha helices and strands, plus a catalytic ferrous ion binding site (Asp178-His180-Asp267). There are four potential splice variants, all of which possess an alternate start site 38 aa upstream of the standard start site. Three have additional changes; two show a one and three aa substitution respectively for aa 211-377, while a third has a 15 aa substitution for aa 148-377. Human HDAC8 shares more than 96% aa identity with both mouse and canine HDAC8.

Overview

Reactivity: Mouse, Rat, Human

Tested applications: Western Blot: 1:1000

Immunohistochemistry: 1:500

Immunoprecipitation: 1:100
ChIP, CUT&RUN and CUT&Tag

Specificity: Recognizes endogenous levels of total HDAC8 protein.

Properties

Immunogen: Recombinant protein within N-terminal human HDAC8 1-150aa

Clonality: Monoclonal, clone number: 2Q7

Isotype: Rabbit IgG

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C

Applications

WB Image: Western Blot: Histone Deacetylase 8/HDAC8 Antibody - Analysis of HDAC8 on different lysates using anti-HDAC8 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: K562image 1 of 9previousnextclose

ICC/IF Image: Immunocytochemistry/Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody - Staining HDAC8 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Anti-HDAC8 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

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