Anti-HIF2 alpha Rabbit mAb

Product

Anti-HIF2 alpha Rabbit mAb

Catalog number :AT0746

Background:

Hypoxia-inducible factor (HIF) is essential for the cellular response to hypoxia. Under normoxia conditions, the α subunit of HIF is ubiquitinated by von Hippel-Lindau (VHL) protein and is degraded in the ubiquitin/proteasome pathway. Hypoxia inhibits the degradation of the α subunit, which leads to its stabilization. HIF, in turn, regulates the transcription of a variety of genes that respond to hypoxia conditions. There are several isoforms of the HIF α subunit. Studies have found that HIF-1α and HIF-2α expression is increased in some human cancers. HIF-1α has both pro- and anti-proliferative activities, whereas HIF-2α does not possess anti-proliferative activity. Therefore, HIF-2α likely plays an important role in tumorigenesis.

UniProt ID: Q99814

Overview

Reactivity: Human, mouse, rat, bovine, Drosophilia melanogaster, Zebrafish

Tested applications: Western Blotting 1:1000

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP) 1:100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody recognizes endogenous levels of total HIF-2α protein.

Properties

Immunogen: a synthetic peptide corresponding to residues surrounding Ala758 of human HIF-2α protein.

Clonality: Monoclonal, clone number: 4B9

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: HIF-2 alpha/EPAS1 Antibody - HIF-2 alpha in human retinal and choroidal primary endothelia lysates using anti-HIF-2 alpha antibody.

Western Blot: HIF-2 alpha/EPAS1 Antibody - HIF2 expression in HIF2 over-expressed HEK 293T cells.

Anti-HIF2 alpha Rabbit mAb

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