Anti-HSD3B2 (3 beta HSD) Rabbit mAb

Product

Anti-HSD3B2 (3 beta HSD) Rabbit mAb

Catalog number :AT0816

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

Expressed in adrenal gland, testis and ovary.

Defects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2) [MIM:201810]. AH2 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: 'salt wasting' (SW, the most severe type), 'simple virilizing' (SV, less severely affected patients), with normal aldosterone biosynthesis, 'non-classic form' or late onset (NC or LOAH), and 'cryptic' (asymptomatic). In AH2, virilization is much less marked or does not occur. AH2 is frequently lethal in early life.

Overview

Reactivity: Mouse, Rat, Human

Tested applications: Western Blotting 1:1000

IHC 1:50-1:500

ICC 1:50-1:500

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody recognizes endogenous levels of total HSD3B2 protein.

Properties

Immunogen: Synthetic peptide corresponding to Human HSD3B2 N terminal.

Clonality: Monoclonal, clone number: 7F2

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at -20°C

Host: Rabbit

Applications

WB Image: Anti-HSD3B2 antibody at 1 :1000 dilution

+ 293T cell lysate at 10 µg

Secondary

HRP conjugated anti-Rabbit IgG at 1:5000 dilution

band size: 42 kDa

ICC/IF Image: ICC/IF image of AT0816 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (AT0816, 1:500) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1:1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HSD3B2 (3 beta HSD) Rabbit mAb

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