Anti-Histone H1.0 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Overview

Description

Anti-Histone H1.0 Rabbit Monoclonal antibody

Reactivity

Human, mouse, rat, bovine, chicken, Drosophilia melanogaster

Tested applications

Western Blotting 1:1000

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP) 1;100

ChIP, CUT&RUN and CUT&Tag

Optimal dilutions/concentrations should be determined by the end user.

Specificity

This antibody detects endogenous levels of total Histone H1.0 protein.

Properties

Immunogen

Recombinant fragment within Human Histone H1.0 aa 1-194. The exact sequence is proprietary.

Clonality

Monoclonal, clone number: 4B7

Isotype

Rabbit IgG

Form

Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Database links

P07305

Host

Rabbit

Applications

WB Image

Western Blot: Histone H1.0 Antibody - Analysis of Histone H1.0 on human lung lysates using anti-Histone H1.0 antibody at 1/1,000 dilution.

IHC Image

Immunohistochemistry-Paraffin: Histone H1.0 Antibody - Analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.

ICC/IF Image

Immunocytochemistry/Immunofluorescence: Histone H1.0 Antibody - Staining Histone H1.0 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.