Anti-Histone H2A.Z (Histone Variant) Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-Histone H2A.Z (Histone Variant) Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :AT0730

Modulation of chromatin structure plays a critical role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regulating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modulate chromatin structure. There are five major variants of histone H2A: canonical H2A (most abundant), H2A.X, MacroH2A, H2ABbd and H2A.Z. Histone H2A.Z, the most conserved variant across species, functions as both a positive and negative regulator of transcription and is important for chromosome stability. Several homologous protein complexes, such as SWR-C (S. cerevisiae), TIP60 (D. melanogaster) and SRCAP (mammals), have been shown to catalyze the ATP-dependent exchange of H2A.Z for H2A in the nucleosome. This exchange of histone H2A variants changes histone-histone interactions in the nucleosome core and alters an acidic patch on the surface of the nucleosome, resulting in changes in nucleosome stability and binding of non-histone proteins such as HP1α.

UniProt ID:

P0C0S5

Overview

Reactivity: Human, mouse, rat, bovine, chicken, Drosophilia melanogaster, Zebrafish

Tested applications: Western Blotting 1:1000

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP) 1;100

Chromatin Immunoprecipitation(ChIP) 1;50
CUT&RUN and CUT&Tag

Optimal dilutions/concentrations should be determined by the end user.

Specificity: Histone H2A.Z Antibody detects endogenous levels of histone H2A.Z protein. The antibody does not cross-react with other histone proteins, including histone H2A.

Properties

Immunogen: Antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human histone H2A.Z. Antibodies are purified by protein A and peptide affinity chromatography.

Clonality: Monoclonal, clone number: 4G32

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: Histone H2A.Z Antibody - Analysis in A375, HEK293, HeLa and SK-MEL-2whole cell lysates.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: Histone H2A.Z Antibody - Staining in HeLa cells (red).

Actin filamentshave been labeled with fluorescein phalloidin (green).

Anti-Histone H2A.Z (Histone Variant) Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

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