Anti-Histone H3.3 (Histone Variant) Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-Histone H3.3 (Histone Variant) Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :AT0731

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

UniProt ID:

P84243

Overview

Reactivity: Human, mouse, rat, bovine, chicken, Drosophilia melanogaster, Zebrafish

Tested applications: Western Blotting 1:1000

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP) 1;100

Chromatin Immunoprecipitation(ChIP) 1;50
CUT&RUN and CUT&Tag

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This Antibody detects endogenous levels of histone H3.3 protein. The antibody does not cross-react with other histone proteins, including histone H3.2 and H3.1.

Properties

Immunogen: Antibodies are produced by immunizing animals with a Synthetic peptide corresponding to Human Histone H3.3. (synthesized peptide derived from Internal of human Histone H3.3.)

Clonality: Monoclonal, clone number: 4B14

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: Histone H3.3 Antibody - Total protein from human HeLa and A431 cells and mouse Neuro2A cells was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1:1000 anti-Histone H3.3 in blocking buffer with an anti-rabbit HRP secondary antibody using chemiluminescence.

IHC Image: Immunohistochemistry-Paraffin: Histone H3.3 Antibody - Analysis of paraffin-embedded mouse stomach tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: Histone H3.3 Antibody - Staining Histone H3.3 in BT-20 cells (green). The nuclear counter stain is DAPI (blue).

Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Anti-Histone H3.3 (Histone Variant) Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

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