Nucleosomes package eukaryotic DNA and are composed of four different histone proteins, designated H3, H4, H2A, and H2B. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. The histone variant H3.3 and the canonical histone H3.1, which differ in only 4- to 5-aa positions, are coexpressed in complex multicellular eukaryotes from fly to human and plant.
H3.1 was enriched in silent areas of the genome, including regions containing the repressive chromatin modifications H3 lysine 27 methylation, H3 lysine 9 methylation, and DNA methylation. In contrast, H3.3 was enriched in actively transcribed genes, especially peaking at the 3' end of genes, and correlated with histone modifications associated with gene activation, such as histone H3 lysine 4 methylation and H2B ubiquitylation, as well as RNA Pol II occupancy.
Anti-Histone H3 Rabbit mAb - Nuclear Loading Control
Catalog number :AT1576
- Overview
- Description
- anti-Histone H3 rabbit monoclonal antibody
- Reactivity
- Human, Mouse, Rat
- Tested applications
- WB(1:1000)
- Properties
- Immunogen
- KLH-coupled synthetic peptide from C-terminal of human Histone H3 (Swiss Prot: P68431)
- Clonality
- Monoclonal, clone number: 12E1
- Isotype
- Rabbit IgG
- Form
- Liquid,100 ug at 1 mg/ml
- Storage instruction
- Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
- Database links
- P68431
- Applications
- Application Image
Anti-Histone H3 antibody (AT1576) at 1/2000 dilution + whole cell lysate at 30 µg
Predicted band size : 15 kDa
Related Products
Reviews
loading...