Anti-Histone H4 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-Histone H4 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :AT0717

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.

UniProt ID:

P62805

Overview

Reactivity: Human, mouse, rat, bovine, chicken, Drosophilia melanogaster, Zebrafish

Tested applications: Western Blotting 1:1000

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP) 1;100
ChIP, CUT&RUN and CUT&Tag

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody recognizes endogenous levels of total histone H4 protein. This antibody does not cross-react with other histone proteins.

Properties

Immunogen: Synthetic peptide corresponding to residues surrounding Ala38 of human histone H4 protein.

Clonality: Monoclonal, clone number: 5C7

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: Histone H4 Antibody - Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE diluted at 1:1000

IHC Image: Immunohistochemistry-Paraffin: Histone H4 Antibody - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: Histone H4 Antibody - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 protein stained by Histone H4 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.

Anti-Histone H4 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

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