Anti-IKK beta Rabbit mAb

Product

Anti-IKK beta Rabbit mAb

Catalog number :AT0363

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.

Overview

Description: Rabbit monoclonal antibody to IKK beta, detects endogenous levels of total IKK beta protein.

Reactivity: Mouse, Human, Rat

Tested applications

WB	: 1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
IHC-P	: Use at an assay dependent dilution.
ELISA	: 1/5000.

Properties

Immunogen: Synthetic non-phosphopeptide derived from human IKK beta around the phosphorylation site of tyrosine 188 (L-Q-Y-L-A).

Clonality: Monoclonal, clone number: 4G1

Isotype: IgG

Form: Liquid
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Storage instruction: Store at -20°C. Stable for 12 months at -20°C

Applications

WB Image: All lanes : Anti-IKK beta antibody (AT0363) at 1/500 dilution

Lane 1 : 293 cell extract, treated with LPS (100ng/ml, 30mins), without the immunizing peptide

Lane 2 : 293 cell extract, treated with LPS (100ng/ml, 30mins), with the immunizing peptide

Predicted band size : 87 kDa

Observed band size : 87 kDa

ICC/IF Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AT0363 (1/50 dilution), in the presence or absence of the immunizing peptide.

Anti-IKK beta Rabbit mAb

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