Anti-JMJD6 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-JMJD6 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :AT0410

Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses.

Overview

Description: Rabbit monoclonal antibody to JMJD6

Reactivity: Mouse, Rat, Human

Tested applications: WB: Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).

ICC/IF: Use a concentration of 1 µg/ml.

ChIP, CUT&RUN and CUT&Tag

Properties

Immunogen: Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human JMJD6.

Clonality: Monoclonal, clone number: 9E7

Isotype: IgG

Form: Liquid
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Database links

Applications

WB Image: All lanes : Anti-JMJD6 antibody (AT0410) at 1 µg/ml

Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Heart (Mouse) Tissue Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 46 kDa
Observed band size : 50 kDa
Additional bands at : 26 kDa,30 kDa,43 kDa (possible isoform). We are unsure as to the identity of these extra bands.

Exposure time : 16 minutes

ICC/IF Image: ICC/IF image of AT0410 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (AT0410, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 4% formaldehyde fixed (10 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.

Anti-JMJD6 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

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