Anti-KMT6 / EZH2 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-KMT6 / EZH2 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :AT1614

The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis. Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain. The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure. Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro, which is thought to be involved in targeting transcriptional regulators to specific loci. Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest.

UniProt ID:

Q15910

Overview

Reactivity: Human, mouse, rat

Tested applications: Western Blotting 1:500-1:1000

Immunofluorescence 1:100

Immunohistochemistry (Paraffin) 1:100
ChIP, CUT&RUN and CUT&Tag

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody recognizes endogenous levels of total Ezh2 protein.

Properties

Immunogen: Synthetic peptide: Human KMT6/ EZH2 aa 350-450

Clonality: Monoclonal, clone number: 6M8

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.4) and 50% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: EZH2/KMT6 Antibody - Analysis of KMT6/EZH2 on different lysates using anti-KMT6/EZH2 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: 293

IHC Image: Immunohistochemistry-Paraffin: EZH2/KMT6 Antibody - Analysis of paraffin-embedded human lung cancer tissue using anti-KMT6/EZH2 antibody. Counter stained with hematoxylin.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: EZH2/KMT6 Antibody - Staining KMT6/EZH2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Anti-KMT6 / EZH2 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

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