Anti-Lamin B1 Mouse mAb - Nuclear Loading Control

Overview

Description

anti- Lamin B1 mouse monoclonal antibody - Nuclear protein loading control

Reactivity

Mouse, Human, Rat

Tested applications

WB(1:1000-1:5000);

Not yet tested in other applications.

Optimal dilutions/concentrations should be determined by the end user. 

Properties

Immunogen

Recombinant fragment: LGKCKAEHDQ LLLNYAKKES DLNGAQIKLR EYEAALNSKD AALATALGDK KSLEGDLEDL KDQIAQLEAS LAAAKKQLAD, corresponding to amino acids 107 - 186 of Human Lamin B1 (NP_005564) with a proprietary tag.

Clonality

7G7

Isotype

Mouse IgG2a

Form

Liquid
Preservative: None
Constituents: 1X PBS, pH 7.2

Storage instruction

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Applications

WB Figure 1

Western Blotting Image

Predicted band size: 70 kDa

 

Anti-Lamin B1 antibody at 1:2000 +Cell  lysates of Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line

 

Recommended secondary antibody: Goat anti mouse IgG-HRP (Engibody, Catalog number:AT0098)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)

WB Figure 2

Western Blotting Image

Predicted band size: 70 kDa

 

Anti-Lamin B1 antibody at 1:5000

Lane 1 (Left): Raw 264.7 cytoplasmic protein at 50 µg 

Lane 2 (Left): NIH3T3 cytoplasmic protein at 50 µg

 

Lane 1 (Right): Raw 264.7 nuclear protein at 50 µg 

Lane 2 (Right): NIH3T3 nuclear protein at 50 µg

 

Recommended secondary antibody: Goat anti mouse IgG-HRP (Engibody, Catalog number:AT0098)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)