MBP-Catch-Mag™ Anti-MBP tag Alpaca Nanobody Conjugated Magnetic Beads

Overview

Description

MBP-Catch-Mag™ Anti-MBP nanobody (amino acid sequence is derived from alpaca VHH, Single Domain antibody) conjugated Magnetic Beads

 

It is recombinantly expressed in yeast.

 

Advantage:

This is the only anti-MBP alpaca nanobody conjugated beads in the world, not Fab conjugated beads from mice or rabbits. This anti-MBP alpaca nanobody is better than Fab due to its higher stability and affinity. More importantly, downstream WB will not be interfered with antibody light and heavy chains.

 

On the contrary, mouse Fab will still be bound by downstream secondary antibodies, resulting in interference with light and heavy chains.

Reactivity

This product is useful tool for IP/CoIP/ChIP of MBP-tagged proteins, and it recognizes MBP-tags placed at N-terminal, C-terminal of the fusion protein.

Tested applications

 

Immunoprecipitation (IP), 

Co-Immunoprecipitation (Co-IP), 

Chromatin Immunoprecipitation (ChIP) 

RNA Binding Protein Immunoprecipitation (RIP)

Enzyme assays

Mass spectrometry

Affinity purification

 

In IP, 25-50 µL of beads suspension for ~500-1000 µL of crude total cell lysate solution.

Optimal dilutions/concentrations should be determined by the end user.

Specificity

This antibody Beads catches MBP-tagged proteins expressed in cells or E. coli. 

Product Picture 1

Figure 1. Alpaca antibodies are smaller than other mammalian antibodies. Mammalian IgG consists a heavy (green) and light (blue) chain, with variable heavy (VH) and variable light (VL) domains that facilitate the memory-like binding properties of antibodies. Alpaca antibodies consist only a heavy chain (green) with a single variable heavy (VHH) domain for target recognition. This VHH domain can be expressed alone without the heavy chains, forming a single domain VHH.

Product Picture 2

Properties

Immunogen

MBP(maltose binding protein) 

Form

Magnetic beads Conjugated anti-MBP nanobody, the product is supplied in 10 mM PBS, pH 7.4, and 0.02% (w/v) sodium azide as preservative.

Storage instruction

Stored at 4 °C. Do not freeze

Beads Diameter

~ 40 µm

Binding Capacity

~1mg MBP tagged protein /ml beads suspension

Applications

Highlights

This antibody is nanobody, amino acid sequence is derived from alpaca VHH, it is recombinantly expressed in yeast.

 

Its advantages are as below:

 

• Fast, reliable & efficient one-step immunoprecipitation

 

• Ready-to-use, high stability, high affinity

 

• No heavy & light antibody chains interference in downstream WB

 

• Stable under harsh washing conditions

 

• Suitable for downstream mass spec, CoIP, ChIP, RIP, protien purification and so on

 

• Works in samples from: mammals, plants, bacteria, yeast, insects etc.