Anti-MGMT Rabbit mAb

Product

Anti-MGMT Rabbit mAb

Catalog number :AT0408

MGMT (O6 methylguanine DNA methyltransferase) is an enzyme that transfers methyl groups from O(6) methylguanine, and other methylated moities of DNA, to a cysteine residue in itself, thus repairing DNA damage caused by alkylation in a single step reaction. This is a suicide reaction as the enzyme is irreversibly inactivated. MGMT prevents mutagenesis and malignant transformation, and also provides resistance to chemotherapy with alkylating agents among cancer patients.

Overview

Description: Rabbit monoclonal antibody to MGMT

Reactivity: Human, Mouse, Rat

Tested applications: WB: 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

ICC/IF: Use at an assay dependent concentration.

ELISA: 1/5000.

Properties

Immunogen: Synthetic peptide derived from the internal region of human MGMT.

Clonality: Monoclonal, clone number: 4B23

Isotype: Rabbit IgG

Form: Liquid
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Storage instruction: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Database links

Applications

WB Image: All lanes : Anti-MGMT antibody (AT0408) at 1/500 dilution

Lane 1 : Extracts from Jurkat cells.
Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg

Lysates/proteins at 5 µg per lane.

Predicted band size : 22 kDa
Observed band size : 22 kDa

IHC Image: AT0408 staining MGMT in T98G human glioma cell line by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 3% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 20°C. The secondary antibody was Alexa Fluor® 555-conjugated goat anti-rabbit polyclonal, diluted 1/300. MGMT staining is shown in red (AF 555) and stains nuclei and to a small extent, cytoplasm. Nuclei were stained with DAPI (blue), while microtubules were stained using an anti-tubulin FITC conjugate (green).

Anti-MGMT Rabbit mAb

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