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    Anti-MMP1 Rabbit mAb

    Catalog number :AT0751
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    Background: 
    Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin,alpha ‑1 antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL-1 beta, IGF-BP3, IGF-BP5, pro MMP-2 and pro MMP-9. A significant role of MMP-1 is the degradation of fibrillar collagens in extracellular matrix remodeling, characterized by the cleavage of the interstitial collagen triple helix into ¾, ¼ fragments. However, as the list of substrates above illustrates, the role of MMP-1 is more diverse than originally envisaged, and may involve enzyme cascades, cytokine regulation and cell surface molecule modulation. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.
     
    UniProt ID: P03956 Human
     
    Overview
    Reactivity
    Human, mouse, rat, bovine, pig
    Tested applications
    Western Blotting 1:1000
    Immunofluorescence  1:500
    Immunohistochemistry (Paraffin) 1:500
    Immunoprecipitation(IP)  1:100 
    Optimal dilutions/concentrations should be determined by the end user. 
     
    Specificity
    This antibody detects pro and active forms of MMP-1. In Western blots, no cross-reactivity with recombinant MMP‑2, MMP‑3, or MMP‑9 is observed.
    Properties
    Immunogen
    Recombinant fragment, corresponding to a region within amino acids 42-244 of Human MMP1.
    Clonality
    Monoclonal, clone number: 4F11
    Isotype
    Rabbit IgG
    Form
    Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol,0.02% Sodium Azide
    Storage instruction
    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.
    Host
    Rabbit
    Applications
    WB Image

    Anti-MMP1 antibody at 1/1000 dilution + Raji whole cell lysate at 30 µg
    Predicted band size: 54 kDa
     
    IHC Image

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse prostate tissue labelling MMP1 with this antibody at a dilution of 1/500.

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