Anti-MMP13 Rabbit mAb

Product

Anti-MMP13 Rabbit mAb

Catalog number :AT0756

Background:

MMP13 (collagenase 3) belongs to the matrix metalloproteinase (MMP) superfamily of enzymes that targets many extracellular proteins, including other proteases, growth factors, cell surface receptors, and adhesion molecules. MMP13 is a member of a subgroup of collagenases (including MMP1, MMP8, and MMP18) that play an even more important function targeting fibrillar collagen. MMP13 is synthesized as a latent proenzyme, and proteolytic removal of the inhibitory propeptide domain is required for enzyme activation. MMP13 protein levels are regulated at the transcriptional level, via specific transcription factors and via promoter DNA methylation. MMP13 preferentially cleaves Type II collagen, and research studies have shown that aberrant upregulation of MMP13 activity can lead to cartilage loss and osteoarthritis. In addition, MMP13 has been shown to promote cancer development, in part through enhancing tumor angiogenesis and metastases, suggesting that collagenase activity may serve as a useful marker of tumor progression.

UniProt ID: P45452

Overview

Reactivity: Human, mouse, rat, bovine, pig

Tested applications: Western Blotting 1:1000

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP) 1:100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody recognizes endogenous levels of total MMP-13 protein.

Properties

Immunogen: a synthetic peptide corresponding to residues surrounding Pro275 of human MMP13 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Clonality: Monoclonal, clone number: 4F13

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: MMP-13 Antibody - Analysis of MMP-13 expression with Jurkat cell lysate.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: MMP-13 Antibody - MMP-13 antibody was tested at 1:50 in MDA-MB-231 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.

Anti-MMP13 Rabbit mAb

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