Anti-Myc tag Mouse mAb (HRP)

Product

Anti-Myc tag Mouse mAb (HRP)

Catalog number :AT0528

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.

Overview

Description: Anti-c-Myc-tag Mouse mAb Conjugated HRP

Reactivity: Myc-Tag Mouse mAb (HRP Conjugated) detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells.

Tested applications: WB : 1/500-1/1000.

Optimal dilutions/concentrations should be determined by the end user.

Product Picture

Properties

Immunogen: Synthetic peptide: C-EQKLISEEDL conjugated to KLH, corresponding to Myc tag.

Isotype: Mouse IgG1

Form: Liquid

pH: 7.40

Preservative: 0.02% Sodium azide

Constituent: PBS

Storage instruction: Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Applications

WB Image: Western Blot: Myc Epitope Tag Antibody - Anti-Myc tag antibody (AT0528) at1:1000,

All lanes: HEK293T Whole Cell Lysate at 50 µg, 40 µg, 30 µg, transfected pCMV-Myc-POI recombinant plasmid

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)

Anti-Myc tag Mouse mAb (HRP)

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