Anti-Myc tag Rabbit mAb

Product

Anti-Myc tag Rabbit mAb

Catalog number :AT0045

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

Overview

Description: Rabbit monoclonal antibody to myc tag

Reactivity: All species

Tested applications: WB : 1/1000-1/5000.

IHC : 1/200-1/500.

ICC/IF : 1/100-1/500.

IP : 1/50-1/200.

Optimal dilutions/concentrations should be determined by the end user.

Product Picture

Properties

Immunogen: EQKLISEEDL(C-MYC) conjugated with KLH.

Clonality: Monoclonal, clone number: 5D12

Isotype: Rabbit IgG

Form: Liquid, 100 ul, at 1mg/ml with PBS, pH 7.4, containing 0.02% sodium azide

Storage instruction: The antibody can be stored for 2-3 weeks at 2-8°C. For long term, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications

Application Image: Western blot

Sample Human Cell lysate - whole cell (293T)

Loading amount 25 µg

Treatment Cells transfected with pCMV-Myc

Gel Running Conditions Reduced Denaturing

Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s)

Recommended secondary antibody: Goat anti rabbit IgG-HRP (Engibody, Catalog number:AT0097)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)

Anti-Myc tag Rabbit mAb

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