Anti-Myc tag Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Overview

Reactivity

All species

Tested applications

IP/CoIP:1/50-1/500
ChIP    : 1/20-1/200.
CUT&RUN and CUT&Tag
 

Optimal dilutions/concentrations should be determined by the end user.

Specificity

c-Myc Antibody Recognizes c-Myc-tagged proteins overexpressed in cells, including both amino- and carboxy-termini of targeted proteins in transfected mammalian cells.

Properties

Immunogen

Synthetic peptide: EQKLISEEDL, corresponding to amino acids 410-419 of Human c-Myc.

Clonality

Monoclonal, clone number:3E12

Isotype

Rabbit IgG

Form

Liquid, 100 μg at 1mg/ml

Storage instruction

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C 

Applications

ChIP Images

A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).