Anti-Nanog Rabbit mAb

Product

Anti-Nanog Rabbit mAb

Catalog number :AT0703

Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells. Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4. Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28.

UniProt ID:

Q80Z64

Overview

Reactivity: Human, mouse, rat, Xenopus laevis, bovine, chicken, Drosophilia melanogaster

Tested applications: Western Blotting 1:1000

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP) 1;100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody detects endogenous levels of total Nanog protein.

Properties

Immunogen: Synthetic peptide corresponding to Human Nanog aa 29-49.

Sequence:

CGPEENYPSLQMSSAEMPHTE

Clonality: Monoclonal, clone number: 6NC7

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: Nanog Antibody

ICC/IF Image: Immunocytochemistry/Immunofluorescence: Nanog Antibody - Nanog antibody was tested in DGCR8 knockout Mouse embryonic stem cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Anti-Nanog Rabbit mAb

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