Anti-Nestin Rabbit mAb

Product

Anti-Nestin Rabbit mAb

Catalog number :AT0707

Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins. It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration. Upon maturation of the brain, nestin expression is quickly down-regulated and replaced by expression of the neurofilament proteins. Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells. Research studies have shown that expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and it is generally accepted as a marker for neural cancer stem cells. However, nestin expression has also been observed in astrocytes, retina, cardiac muscle, pancreas, and other tissues. Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous.

UniProt ID:

P48681

Overview

Reactivity: Human, mouse, rat, bovine, chicken, Drosophilia melanogaster

Tested applications: Western Blotting 1:1000

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP) 1;100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody detects endogenous levels of total Nestin protein.

Properties

Immunogen: Full length native protein (purified) corresponding to Rat Nestin.

Clonality: Monoclonal, clone number: 5A1

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: Nestin Antibody –

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10

Lane 2: Human cell line SK-MEL-30

IHC Image: Immunohistochemistry-Paraffin: Nestin Antibody - Staining of human kidney shows high expression

ICC/IF Image: Immunocytochemistry/Immunofluorescence: Nestin Antibody - Staining of human cell line U-251 MG shows localization to intermediate filaments. Antibody staining is shown in green.

Anti-Nestin Rabbit mAb

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