Ni-NTA Magnetic Beads for His-tagged Protein Purification
Catalog number :P0030
Magit™ Ni-NTA Magnetic Beads are used for small scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). These tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates. Purification of His-tagged proteins is achieved using a NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites which allow for low metal ion leaching and high binding capacity. The protocol for the Ni-NTA Magnetic Beads has been optimized to allow for high purity of the isolated His-tagged protein. Performance is equivalent to or better than Ni-NTA magnetic beads from other suppliers.
- Overview
- Description
- Ni-NTA Magnetic Beads for His-tagged Fusion Protein Purification
- Tested applications
- His-tagged Fusion Protein Purification
- Product Picture
- Properties
- Form
- 20% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol.
- Storage instruction
- Store at 4℃, DO NOT freeze.
- Conjugation
- Nickel loaded on nitrilotriacetic acid that has been covalently coupled to a blocked magnetic bead surface
- Structure Image
- Beads Diameter
- average 40 μm
- Binding Capacity
- more than 3 to 4 mg His tagged fusion protein per 1 ml 20% slurry
- Applications
- Application Image
- Highlights
- Extremely low nonspecific binding
Magnetic Beads can be regenerated for multiple uses
High capacity, equivalent or higher binding capacity than Ni-NTA magnetic beads from other suppliers
- Work Flow Image
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