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    Ni-NTA Magnetic Beads for His-tagged Protein Purification

    Catalog number :P0030
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    Magit™ Ni-NTA Magnetic Beads are used for small scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). These tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates. Purification of His-tagged proteins is achieved using a NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites which allow for low metal ion leaching and high binding capacity. The protocol for the Ni-NTA Magnetic Beads has been optimized to allow for high purity of the isolated His-tagged protein. Performance is equivalent to or better than Ni-NTA magnetic beads from other suppliers.
    Overview
    Description
    Ni-NTA Magnetic Beads for His-tagged Fusion Protein Purification
    Tested applications
    His-tagged Fusion Protein Purification
    Product Picture
    Ni-NTA Magnetic Beads for His-tagged Protein Purification
    Properties
    Form
    20% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol.
    Storage instruction
    Store at 4℃, DO NOT freeze.
    Conjugation
    Nickel loaded on nitrilotriacetic acid that has been covalently coupled to a blocked magnetic bead surface
    Structure Image
    Beads Diameter
    average 40 μm
    Binding Capacity
    more than 3 to 4 mg His tagged fusion protein per 1 ml 20% slurry
    Applications
    Application Image
    Highlights
    Extremely low nonspecific binding
    Magnetic Beads can be regenerated for multiple uses
    High capacity, equivalent or higher binding capacity than Ni-NTA magnetic beads from other suppliers
    Work Flow Image
    Ni-NTA Magnetic Beads for His-tagged Protein Purification

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