Ni-NTA Agarose Resin 6FF Prepacked Column for His Protein Purification

Overview

Tested applications

Medium- to large-scale FPLC purification of polyhistidine tagged proteins

Properties

Form

Prepacked Column

Storage instruction

2 to 8°C, 

Up to 25 times

Conjugation

Metal: ≥15μmol Ni2+/mL resin

Structure Image

Beads Diameter

90 μm

Binding Capacity

Static: approx. 60mg 6xHis-GFP/mL resin

Matrix

Superflow 6 Resin, highly crosslinked 6% sepharose

Chemical stability

1M acetic acid pH 2, 1% SDS pH 7, 6M guanidine-HCl, 70% ethanol for ≥1 week at 37°C; 8M urea, 10mM DTT, 5mM TCEP for ≥2 hours at 22°C

Flow rate

Recommended: 300 cm/hour (binding, wash, elution)

pH stability

pH 3 to 9 for ≥1 week at 4°C

Storage buffer

20% ethanol

Applications

Highlights

High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour

High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates

Versatile – can be used to purify proteins under native and denaturing conditions

Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour

Compatible – maintains function in a wide variety of chemicals and pH conditions

Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse