Ni charged Magnetic Beads for His Pull-Down Assay

Catalog number :IF9301

• Discover a new protein:protein interaction from a cell lysate

• Confirm a putative interaction from a cell lysate or with a previously purified protein

• Extract protein:protein interaction information from in vitro transcription/translation lysates

Overview

Tested applications: His Pull-Down Assay.

Optimal amount should be determined by the end user.

Properties

Form: Magnetic Beads in Suspension (20% slurry), supplied in phosphate buffered saline (PBS) pH 7.4, with 0.02% sodium azide as preservatives.

Storage instruction: Store at 4°C. This product is stable for 12 months. Do not freeze, dry or centrifuge magnetic beads. This may cause irreversible aggregation and decreased binding capacity.

Bead Diameter:	40 µm
Concentration:	20% slurry
Binding Capacity:	40 to 50 mg His fusion protein per 1 mL settled beads (5mL 20% slurry).

Applications

Protocols: Procedure Summary for His Pull-Down Assay

I. Preparation of Bait Protein

Protocol 1: Bait from E. coli Expression Systems

Protocol 2: Bait from Insect Cell Expression Systems

Protocol 3: Bait from Previously Purified Protein

II. Immobilization of Bait Protein

A. Preparation of Ni charged beads

B. Immobilize Bait Protein

III. Preparation of Prey Protein

Protocol 1: Prey Protein from E. coli or Baculovirus-Infected Insect Cell Lysate

Protocol 2: Prey Protein from Mammalian Cell Lysate

Protocol 3: Prey from Previously Purified Protein

Protocol 4: Prey Protein from In vitro Transcription/Translation Reaction

IV. Capture of Prey Protein

V. Elution of Bait-Prey

VI. SDS PAGE Gel Analysis or WB Analysis