Anti-Notch1 Rabbit mAb

Product

Anti-Notch1 Rabbit mAb

Catalog number :AT0697

Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate. Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes.

UniProt ID:

P46531

Alternate Names for Notch1 Antibody:

EC 2.1.2.11, EC 3.4.21.68, hN1, Notch (Drosophila) homolog 1 (translocation-associated), notch 1Notch homolog 1, translocation-associated (Drosophila), Notch homolog 1, translocation-associated

Notch-1, TAN1, TAN1neurogenic locus notch homolog protein 1, Translocation-associated notch protein TAN-1

Overview

Reactivity: Human, mouse, rat, Xenopus laevis, bovine, chicken, Drosophilia melanogaster

Tested applications: Western Blotting 1:1000

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody detects intracellular epitopes between 2400 and 2500 amino acids of human Notch1. It recognizes both the full-length (~300 KDa) and the NTM region (~120 KDa), which consists of a short extracellular juxtamembrane peptide, a transmembrane sequence and the intracellular domain (NICD). The antibody cannot detect the extracellular (ligand-binding) domain of Notch1 following cleavage at the S2 site by ADAM-type metalloproteases.

Properties

Immunogen: Synthetic peptide within Human Notch1 aa 2400-2500. The exact sequence is proprietary.

Clonality: Monoclonal, clone number: 6D7

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: Notch-1 Antibody - WB analysis of Cleaved Notch1 in Jurkat cell lysate.

IHC Image: Immunohistochemistry: Notch-1 Antibody - IHC staining of Notch1 in human kidney carcinoma using DAB with hematoxylin counterstain.

Anti-Notch1 Rabbit mAb

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