Anti-Oct4 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-Oct4 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :AT0699

Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells. A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation. Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker. Oct-4 exists as two splice variants, Oct-4A and Oct-4B. Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells.

UniProt ID:

Q01860

Overview

Reactivity: Human, mouse, rat, Xenopus laevis, bovine, chicken, Drosophilia melanogaster

Tested applications: Western Blotting 1:1000

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP) 1;100

ChIP, CUT&RUN and CUT&Tag

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody detects endogenous levels of total Oct-4 protein.

Properties

Immunogen: Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Oct4.

Clonality: Monoclonal, clone number: 8DN7

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: OCT4 Antibody - Sample (10 ug of whole cell lysate) A: Non-transfected HeLa lysates B: OCT 3/4 transfected HeLa lysates 10% SDS PAGE; antibody diluted at 1:2000.

Anti-Oct4 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

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