Glutathione (Reduced GSH) for Elution
Catalog number :P5515
Glutathione is a tripeptide comprised of three amino acids (cysteine, glutamic acid, and glycine) present in most mammalian tissue. Glutathione acts as an antioxidant, a free radical scavenger and a detoxifying agent. Glutathione is also important as a cofactor for the enzyme glutathione peroxidase, in the uptake of amino acids, and in the synthesis of leukotrienes. As a substrate for glutathione S-transferase, this agent reacts with a number of harmful chemical species, such as halides, epoxides and free radicals, to form harmless inactive products. In erythrocytes, these reactions prevent oxidative damage through the reduction of methemoglobin and peroxides. Glutathione is also involved in the formation and maintenance of disulfide bonds in proteins and in the transport of amino acids across cell membranes.
- Overview
- Description
L-Glutathione (Reduced GSH) for Elution
- Tested applications
- Competitive Elution of GST tagged protein from GSH beads (GSH agarose beads or GSH magnetic beads)
L-Glutathione (GSH) reduced has been used in the elution buffer to elute GST (glutathione S-transferase)-fused proteins using glutathione-agarose beads. It has been used to prepare a standard curve for GSH analyses.May be used at 5-10 mM to elute glutathione S-transferase (GST) from glutathione agarose.
- Properties
- Form
- Powder
- Storage instruction
- Room temperature
- Structure Figure 1
- Structure Figure 2
- Structure Figure 3
- CAS #
- 70-18-8
- Molecular weight
- 307.33 g/mol
- Purity
- > 99%
- Molecular Formula
- H2NCH(CO2H)CH2CH2CONHCH(CH2SH)CONHCH2CO2H
- Amino Acid Sequence
- γ-Glu-Cys-Gly
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