CleaTag™ SUMO Protease

Overview

Description

CleaTag™ SUMO Protease(Recombinant, his-tagged)

Properties

Form

N-terminal 2*polyhistidine tag, yeast SUMO protease variant corresponding to the active SUMO protease domain (amino acids Leu403-Lys621) expressed recombinantly in and purified from Yeast

Storage instruction

Shipped in Dry ice
Upon receipt, store SUMO protease at -80℃ and buffers at -20℃. Aliquot after the first thawing. Please avoid repeated freeze/thaw cycles. Stable for 24 months when stored properly.

Database links

Uniprot: Q02724 (ULP1_YEAST)

Storage buffer

12.5 mM Tris (pH 7.5), 250 mM NaCl, 125 mM Imidazole, 1 mM DTT, 50 % glycerol

Synonyms

Yeast SUMO protease,Ubiquitin-like-specific protease 1,Small Ubiquitin-like Modifier Protease,Ulp1

Molecular weight

28kDa

Specific activity

Cutting will be dependent on spacing between the SUMO tag and the rest of the protein. Hence, it is hard to define activity. In most cases, 1 hour incubation at a ratio 1:200 at room temperature will result in >99% cleavage of the SUMO tag.

Purity

>95%

Applications

Application Image

Protein X was expressed as a SUMO fusion protein. To remove the SUMO tag, the SUMO-X fusion protein was incubated with SUMO protease at a ratio of 200:1 (w:w); after 10 minutes, >99% of the protein was cleaved.

Highlights

• Highly active cleavage

• No non-specific proteolysis (highly specific cleavage)

• Activity from 2°C to 37°C

• A six-histidine sequence to facilitate its removal from the digested protein sample

Application Figure 1