Alkali-Tolerant Protein A agarose Resin 6FF for Antibody Purification

Product

Alkali-Tolerant Protein A agarose Resin 6FF for Antibody Purification

Catalog number :P5555

rProtein A Agarose 6 Fast Flow is a proprietary affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably monoclonal antibodies, from samples of larger volumes.

Alkali-tolerant protein A is engineered by gene recombination technology on the base of native protein A. Our Alkali-Tolerant Protein A Agarose 4FF retains ≥95% capacity after 200 times repeated clean-in-place (CIP) cycles with 0.1M NaOH, and approximately 80% of the initial capacity with 0.5M NaOH after 100 times repeated CIP cycles. Alkali-Tolerant Protein A is immobilized onto the cross-linked 6% agarose resin with an orientation that is optimal for immunoglobulin binding. The level of leakage of the ligand during elution is very low.

Overview

Description: Alkali-Tolerant Protein A agarose Resin 6FF for Antibody Purification

Tested applications: Antibody Purification

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Specificity

Properties

Form: 50% Slurry

Storage instruction: Store at 2 - 8°C. Do not Freeze

Beads Diameter: an average of ~ 90 μm

Binding Capacity: >40 mg human IgG/mL settled resin

Ligand: Recombinant alkali-tolerant protein A produced in E.coli

Matrix: Highly cross-linked 6% agarose beads

Maximum pressure: 0.3 MPa (3 bar)

Chemical stability: All commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea.

Flow rate: Gravity flow and medium pressure, 30 - 300 cm/hour

Storage buffer: 1x PBS containing 20% ethanol

Cleaning-in-place: 0.1 M ~ 0.5 M NaOH

Applications

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Alkali-Tolerant Protein A agarose Resin 6FF for Antibody Purification

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