Alkali-Tolerant Protein A agarose Resin 6FF Prepacked Column for Antibody Purification
Catalog number :P5556
Alkali-tolerant protein A is engineered by gene recombination technology on the base of native protein A. Our Alkali-Tolerant Protein A Agarose 6FF retains ≥95% capacity after 200 times repeated clean-in-place (CIP) cycles with 0.1M NaOH, and approximately 80% of the initial capacity with 0.5M NaOH after 100 times repeated CIP cycles. Alkali-Tolerant Protein A is immobilized onto the cross-linked 6% agarose resin with an orientation that is optimal for immunoglobulin binding. The level of leakage of the ligand during elution is very low.
- Overview
- Description
- Alkali-Tolerant Protein A agarose Resin 6FF Prepacked Column for AKTA
- Tested applications
- Antibody Purification
- Specificity
- Properties
- Form
- Prepacked Column
- Storage instruction
- store at 2 - 8°C. Do not Freeze.
- Structure Image
- Beads Diameter
- an average of ~ 90 μm
- Binding Capacity
- >40 mg human IgG/mL settled resin
- Ligand
- Recombinant alkali-tolerant protein A produced in E.coli
- Matrix
- Highly cross-linked 6% agarose beads
- Maximum pressure
- 0.3 MPa (3 bar)
- Chemical stability
- All commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea.
- Flow rate
- Gravity flow and medium pressure, 30 - 300 cm/hour
- Cleaning-in-place
- 0.1 M ~ 0.5 M NaOH
- Applications
- Application Image
- Application Figure 1
- Application Figure 2
- Application Figure 3
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