Anti-PARP1 Rabbit mAb

Product

Anti-PARP1 Rabbit mAb

Catalog number :AT0396

PARP, a 114 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis

Overview

Description: Rabbit Monoclonal antibody to PARP1

Reactivity: Human; Mouse; monkey; dog; rabbit; Chinese hamster

Tested applications: WB: 1/1000 - 1/2000; IF: 1/100-1/500

Properties

Immunogen: This antibody is produced by immunizing rabbit with a polypeptides corresponding to surface epitope of human PARP1

Clonality: Monoclonal, clone number: 5G1

Isotype: Rabbit Polyclonal

Form: Liquid
Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, PBS

Storage instruction: Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Database links

Applications

WB Image: All lanes: Anti-PARP1 antibody at 1/2000 dilution

Lane 1: Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract

Lane 2: PARP1 shRNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract

Lysates/proteins at 30 µg per lane.

Secondary

All lanes: HRP-conjugated anti-rabbit IgG

Predicted band size: 113 kDa

IHC Image: Paraffin-embedded HeLa xenograft tissue stained for PARP1 using this antibody at 1/500 dilution in immunohistochemical analysis.

IP/CoIP Image: PARP1 was immunoprecipitated from HCT 116 (human colorectal carcinoma cell line) whole cell extract with 4 µg this antibody. Western blot was performed from the immunoprecipitation using this antibody at 1/500 dilution. Anti-Rabbit IgG was used as a secondary reagent.

Lane 1: HCT 116 whole cell extract 30 μg.

Lane 2: Control IP in HCT 116 whole cell extract with 4 μg of Rabbit IgG.

Lane 3: This antibody IP in HCT 116 whole cell extract.

Anti-PARP1 Rabbit mAb

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