RFP-Alpaca-Nano™ Anti-RFP tag Alpaca Nanobody(VHH) (unconjugated) is designed for detecting RFP tag fusion proteins specifically. It is recombinant alpaca nanobody(VHH). It detects the RFP tag selectively, no reactivity with other proteins.
Red fluorescent protein cloned from Discosoma coral (DsRed or drFP583)is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish).
RFP-Alpaca-Nano™ Anti-RFP tag Alpaca Nanobody(VHH)
Catalog number :AT8310
- Overview
- Description
- RFP-Alpaca-Nano™ Anti-RFP tag Alpaca Nanobody(VHH)
- Reactivity
- Detect transfected RFP tag fusion proteins expressed in E. coli, yeast, insect cell, mammalian cell, or in vitro transcription/translation systems. All systems that express RFP tag fusion protein are suitable.
- Properties
- Immunogen
- Recombinant full length RFP.
- Clonality
- Alpaca monoclonal nanobody(VHH,single domain antibody), without Fc fusion
- Form
- Liquid
- Storage instruction
- Store at –20 °C, (Avoid freeze / thaw cycles)
- Conjugation
- Unconjugated
- Host
- Alpaca pacous
- Storage buffer
- In 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol with 0.02% (w/v) ProClin as preservative.
- Purity
- Recombinant expression in yeast and affinity purified
- Applications
- Highlights
- Benefits Of Alpaca nanobodyNo Fc fragment of ordinary antibodies, so there is no non-specific binding of Fc to the cell surface Fc receptor of immune cells in flow cytometry experimentsHighly specific bindingLow backgroundHigh affinityHigh stabilityAlpaca nanobody is a small molecule with molecular weight of only 13kD (Conventional rabbit or mouse antibody is 150kD), so it can easily enter permeablized cellsAnimal-free production, recombinant expression in yeast and affinity purified
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